The penetration of antibiotics in necrotic tuberculosis lesions is heterogeneous and drug-specific, but the factors underlying such differential partitioning are unknown. We hypothesized that drug binding to macromolecules in necrotic foci (or caseum) prevents passive drug diffusion through avascular caseum, a critical site of infection. Using a caseum binding assay and MALDI mass spectrometry imaging of tuberculosis drugs, we showed that binding to caseum inversely correlates with passive diffusion into the necrotic core. We developed a high throughput assay relying on rapid equilibrium dialysis and a caseum surrogate designed to mimic the composition of native caseum. A set of 279 compounds was profiled in this assay to generate a large dataset and explore the physicochemical drivers of free diffusion into caseum. Principle component analysis and modeling of the dataset delivered an in silico signature predictive of caseum binding, combining 69 molecular descriptors. Among the major positive drivers of binding were high lipophilicity and poor solubility. Determinants of molecular shape such as the number of rings – particularly aromatic rings – number of sp2 carbon counts and volume-to-surface ratio negatively correlated with the free fraction, indicating that low molecular weight non-flat compounds are more likely to exhibit low caseum binding properties and diffuse effectively through caseum. To provide simple guidance in property-based design of new compounds, a rule-of-thumb was derived whereby the sum of hydrophobicity (cLogP) and aromatic ring count is proportional to caseum binding. These tools can be used to ensure desirable lesion partitioning and guide the selection of optimal regimens against tuberculosis.
22 Jun 2016